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2016FallSymposiumURCreativityEngagement has ended
Tuesday, December 6 • 8:00am - 8:20am
Subspecies Determination In Hellbenders (Cryptobranchus Alleganiensis) By The Cytb Gene

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Hellbenders (Cryptobranchus alleganiensis) are large aquatic salamanders endemic to eastern North America that are currently in decline due to habitat loss. The Eastern Hellbender (Cryptobranhus alleganiensis alleganiensis) is classified as near threatened and the Ozark Hellbender (Cryptobranchus alleganiensis bishopi) is classified as endangered. Due to the phenotypic similarities of the two subspecies DNA sequencing was performed and analyzed in order to determine which species is at the Western North Carolina Nature Center. DNA was extracted from a sample of slime provided by the WNC Nature Center. For this study, we performed genetic sequencing of the Cytb gene, which was analyzed to determine if the species of hellbender at the WNC Nature Center was an Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) or the critically endangered Ozark subspecies (Cryptobranchus alleganiensis bishopi). The Cytb gene was amplified via PCR, sequenced, and compared to published hellbender sequences for subspecies determination.


Tuesday December 6, 2016 8:00am - 8:20am
038 Karpen Hall

Attendees (1)