2016FallSymposiumURCreativityEngagement has ended
Tuesday, December 6 • 8:40am - 9:00am
Ectomycorrhizal Fungi On Quercus Seedlings In The Southern Appalachian Mountains

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Oaks in the southern Appalachians play an important role in forest ecology. Because of their ecological importance and the difficulty of regenerating oaks in forests, oak regeneration has been a much researched topic. Ectomycorrhizal fungi are critical to the growth and survival of oaks (Quercus spp), so understanding mycorrhizal associations could be critical to their conservation especially under the increasing threat of pests. Some fungi form symbiotic relationships with multiple host species, others form species specific relationships. The composition of fungal species on host trees is influenced by the biotic and abiotic environments. This study examined the diversity of ectomycorrhizal species on three oak seedlings harvested from the Bent Creek Experimental Forest (Buncombe County, NC). Seedlings were cultivated under different conditions affecting mycorrhizal relationships: 1) with trenching to exclude root competition and common mycorrhizal networks (CMNs) 2) in mesh bags to exclude root competition while allowing access to CMNs and 3) no treatment. Seedlings were harvested and ectomycorrhizal root tips were visually identified and removed. DNA was extracted from the root tips, amplified via PCR, and sequenced. DNA sequences were used to identify the species of fungi present on the seedlings, offering insight and knowledge regarding the identity of ectomycorrhizal symbionts on oak trees at Bent Creek.

Tuesday December 6, 2016 8:40am - 9:00am
038 Karpen Hall