2016FallSymposiumURCreativityEngagement has ended
Tuesday, December 6 • 10:15am - 10:35am
Survey Of Culturable Bacterial Communities Found In Sarracenia Purpurea Var. Montana In Western North Carolina.

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Sarracenia purpurea var. montana is a rare subspecies of the carnivorous purple pitcher plant located within limited habitats in North Carolina bogs. Pitcher plants have tubular leaves that form pitchers which collect water. In this fluid, there is a diverse community of symbiotic organisms, including bacteria and invertebrates. To identify the bacterial species in these carnivorous plants, fourteen fluid samples from Sarracenia purpurea var. montana pitchers from different individual plants were collected. Sample sites were Dupont State Forest and Sherwood Forest in Western North Carolina. The collected bacteria were grown, DNA was extracted, and bacterial 16s rRNA was amplified using PCR. Sanger sequencing was conducted on the PCR products. Species were identified by comparing these results to previously published DNA sequences.

Tuesday December 6, 2016 10:15am - 10:35am
038 Karpen Hall