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2016FallSymposiumURCreativityEngagement has ended
Tuesday, December 6 • 9:20am - 9:40am
Identification Of Gray Wolf (Canis Lupus) Pup’s Ancestral Geographic Origin Using Mitochondrial DNA

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The gray wolf species (Canis lupus) has been divided into two classifications, New World subspecies (C. lupus lycaon) and Old World subspecies (C. lupus). The New World subspecies, otherwise known as the Eastern Wolf, is listed on the endangered species list, and was thought to have evolved from hybridization between gray wolves (C. lupus) and red wolves (C. rufus). Further examination showing that the pup’s grandparents were also of New World decent would call for increased protection of the pups. This cannot be identified phenotypically though, and thus DNA sequencing analysis must be utilized. Mitochondrial DNA was extracted from blood samples provided by the WNC Nature Center and the D-loop (control region) was amplified and then sequenced. DNA sequences were compared to other published sequences to determine the ancestral lineage of the wolves.


Tuesday December 6, 2016 9:20am - 9:40am
038 Karpen Hall