2016FallSymposiumURCreativityEngagement has ended
Tuesday, December 6 • 9:00am - 9:20am
Effect Of Testosterone, 5-Dihydrotestosterone, Mouse Growth Hormone, And Thyroxine On Major Urinary Protein (MUP) Expression In Female Murine Hepatocyte Cell Line Hepa1-6

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Many organisms use chemicals such as pheromones to carry out interspecies communication. In mice, the Major Urinary Proteins (MUPs) serve as pheromones informing many behaviors, including aggression, mate attraction and territory marking. Out of the 21 possible MUP sequences within the mouse genome, only a subset of these proteins is expressed, allowing each individual to produce its own identifiable signature of expressed proteins in detectable concentrations. However, the mechanisms governing which MUPs are expressed by an individual remains unknown. This study aims to explore the mechanisms driving gene expression of the 21 MUP genes. By treating the cultured female murine hepatocyte cell line Hepa1-6 with testosterone, 5-dihydrotestosterone (DHT), mouse growth hormone (mGH), and thyroxine (T4), MUP expression can be induced in a culture dish, allowing us to simulate the natural production of proteins. After inducing MUP expression through hormone treatments, expressed MUPs can be identified through sequencing, and additional tests such as western blot analyses and comparative analyses of epigenetic modifications between expressed and unexpressed proteins can be done to show the stage of translation or transcription at which MUP expression and gene choice are being regulated. These results will demonstrate the genetic mechanisms dictating identity and social interaction between mice, providing broader insight into gene expression as a whole.


Tuesday December 6, 2016 9:00am - 9:20am
038 Karpen Hall