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Tuesday, December 6 • 1:40pm - 2:00pm
Determining The Origin Of A Recently Discovered Local Population Of Rosyside Dace (Clinostomus Funduloides)

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The rosyside dace (Clinostomus funduloides) is a common minnow in headwaters and streams throughout the eastern United States, including most of the Appalachian region. This species, however, has only recently been found in local headwaters of the Upper French Broad River basin. It is unknown whether this population was evolutionarily isolated from the nearby Catawba River basin, or if the species has recently arrived as a result of human activity, such as bait bucket introduction. To answer this question, we first isolated DNA from livers and muscles of fish collected in the Upper French Broad and Catawba headwaters. We then PCR-amplified the cytochrome-b gene, a highly conserved region of mitochondrial DNA, for each specimen. Next, we sequenced PCR products, then compared sequences via GenBank BLAST to determine percent similarity between regions. Given that the Upper French Broad population is a recent discovery despite a consistent history of regional wildlife surveys, we expect that DNA comparison will provide evidence that the species was recently introduced by translocation of fish from the Catawba or another nearby river system. This is the first known study of the Upper French Broad rosyside dace population, and will provide valuable data supporting regional wildlife surveys and conservation efforts.

Tuesday December 6, 2016 1:40pm - 2:00pm PST
038 Karpen Hall