2016FallSymposiumURCreativityEngagement has ended
Tuesday, December 6 • 10:15am - 12:00pm
Developing Patch Clamp Analysis Techniques To Deorphanize VSNRs

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Major urinary proteins (MUPs) are a class of species-specific pheromones that have been found to produce consistent and specific behavioral responses in mice independent of prior conditioning. MUPs are detected through an accessory olfactory system known as the vomeronasal organ, which expresses sensory receptors (VSNRs) designed for MUP binding. Here the activity of VNSRs is intended to be studied using patch clamp analysis by recording VNSR activation upon exposure to specific MUPs as evidenced by voltage change. HEK 293 cells will be transfected to express VNSRs for analysis. The current primary goal includes developing efficient transfection protocol and patch clamp procedure. This research is proposed to allow for the future tracing of synaptic projections throughout the brain beginning with VSNR activation, providing insight into the neurological basis of behavioral reactions.

Tuesday December 6, 2016 10:15am - 12:00pm
Wilma Sherrill Center - Concourse