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2016FallSymposiumURCreativityEngagement has ended
Tuesday, December 6 • 9:00am - 9:40am
Isolation and characterization of antibiotic compounds produced by Gram-Negative bacteria strains Pseudomo nas and C hromobacterium

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Multidrug resistant bacterial infections, which arise due to misuse and overuse of antibiotics, are responsible for many nosocomial infections and are a threat to human health. Derivatization of known antibiotic compounds via total or semisynthesis can be time consuming and ineffective at targeting specific bacteria. This investigation focuses on bacteria found in the phytotelmata of Sarracenia pitcher plants and the natural antibiotic compounds they secrete under varying conditions The aim of the project is to find single-producer and co-culture producing bacteria that secrete secondary metabolites effective a broad spectrum of Gram-positive and -negative pathogens. A Pseudomonas (CMCP E3) and Chromobacterium (CP2 SSIV)isolated in this study have been found to be effective against Gram-positive bacteria Sta phylococcus aureus and the fungi Fusarium solani. The bacteria strains were cultured in minimal media containing either succinate or citrate that showed the densest growth after 72 hours. A 6 L citrate culture of the CMCP E3 bacterium yielded on average 32 mg of crude product and 9 mg of antibacterial compound. Optimization of large scale culture of CP2 SSIV and characterization of the antibacterial secondary metabolite produced by CMCP E3 using NMR and mass spectrometry are ongoing.

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Tuesday December 6, 2016 9:00am - 9:40am
014 Zeis Hall